The EU's foreign policy chief says there is "full EU unity" in support of Jerusalem becoming the capital of both Israel and a future Palestinian state.
Federica Mogherini said the bloc's member states would not recognise the city as Israel's capital before a final status peace agreement.
She spoke after meeting Israeli Prime Minister Benjamin Netanyahu, who wants the EU to follow the US in doing so.
President Donald Trump's move prompted widespread international criticism.
Ms Mogherini, who was speaking at a news conference in Brussels alongside Mr Netanyahu, said the EU would continue to recognise the "international consensus" on Jerusalem.
Mr Netanyahu is in Brussels for talks with EU foreign ministers - the first time an Israeli prime minister has visited the city in more than 20 years.
Mr Trump's announcement drew worldwide condemnation and sparked protests which continued for a fifth day on Monday.
As well as recognising Jerusalem, President Trump also said he was directing the US state department to prepare to move the US embassy from Tel Aviv to Jerusalem, reports BBC.