Theresa May has launched her strongest attack on Russia yet, accusing Moscow of meddling in elections and carrying out cyber espionage.
Addressing leading business figures at a banquet in London, the prime minister said Vladimir Putin's government was trying to "undermine free societies".
Mrs May said it was "planting fake stories" to "sow discord in the West".
But, she added, whilst the UK did not want "perpetual confrontation" with Russia, it would protect its interests.
Her comments are in stark contrast to those of US President Donald Trump, who last week said he believed President Putin's denial of intervening in the 2016 presidential election.
Foreign Secretary Boris Johnson is due to visit Russia next month.
In a major foreign policy speech at the Lord Mayor's Banquet at Mansion House, which Mrs May described as a "very simple message" for President Putin, she said he must choose a very "different path" from the one that in recent years had seen Moscow annex Crimea, foment conflict in Ukraine and launch cyber attacks on governments and Parliaments across Europe.
Russia "Russia has repeatedly violated the national airspace of several European countries and mounted a sustained campaign of cyber espionage and disruption.
"This has included meddling in elections and hacking the Danish Ministry of Defence and the Bundestag among many others."
"We know what you are doing and you will not succeed. Because you underestimate the resilience of our democracies, the enduring attraction of free and open societies and the commitment of Western nations to the alliances that bind us."
She said as the UK left the EU and charted a new course in the world, it remained absolutely committed to Nato and securing a Brexit deal which "strengthens our liberal values", adding that a strong economic partnership between the UK and EU would be a bulwark against Russian agitation in Europe.
Mr Johnson, who will be making his first trip to Russia since becoming foreign secretary in December, has said the UK's policy to Russia must be one of "beware but engage" following a decade of strained relations.